Monthly Archives: October 2014

H and E Part One: History, Background and Solutions

In the previous articles we looked at tissue preparation, sectioning and making slides. Now that you have slides ready for use, the next stage is immunohistochemistry or staining. One of the most commonly used stains for examining tissue is haematoxylin …

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A Slice (or Two) of Life: Cutting Good Sections

Assuming you have fixed, processed and embedded your tissue correctly, the next stage in the life of a paraffin block is to cut good sections. As anyone who has used a microtome will attest, this isn’t always the easiest thing …

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A Ride on the Carousel! What Happens to Tissue During Histological Processing?

Between the fixation of tissue and the embedding in paraffin blocks, tissue is ‘processed’. You can’t embed fixed tissue directly into paraffin wax, so what exactly is tissue processing and what happens in all of the stages? Read on! After …

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Posted in General