Monthly Archives: December 2014

Unmask Those Antigens: Retrieval Techniques to Enhance Immunohistochemistry

If you recall the previous article on fixation of tissue, you will remember that the process of paraformaldehyde (PFA) fixation involves the cross-linking of proteins. This has the obvious advantage of preserving the ‘architecture’ of the tissue as covalent bonds …

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H and E Part Two: Method, Tips and Troubleshooting

The complete method for H&E staining is contained in the tables below, but we’ll take a look at each of the stages in turn and explain the process which should help you should you need to troubleshoot.   Dewax and …

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Posted in General