If you recall the previous article on fixation of tissue, you will remember that the process of paraformaldehyde (PFA) fixation involves the cross-linking of proteins. This has the obvious advantage of preserving the ‘architecture’ of the tissue as covalent bonds …

The complete method for H&E staining is contained in the tables below, but we’ll take a look at each of the stages in turn and explain the process which should help you should you need to troubleshoot.   Dewax and …